Mini Samples ELISA Kit for Fibroblast Growth Factor 6 (FGF6)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than

Sample type

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Fibroblast Growth Factor 6 (FGF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Fibroblast Growth Factor 6 (FGF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Fibroblast Growth Factor 6 (FGF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Fibroblast Growth Factor 6 (FGF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

HBGF6; HST2; HST2 Oncogene; Heparin Secretory-Transforming Protein 2; Heparin-Binding Growth Factor 6

Item Name

Fibroblast Growth Factor 6

Research Area

Cytokine;Tumor immunity;Infection immunity;

Reference