Mini Samples ELISA Kit for Estradiol (E2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.15pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Estradiol (E2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Estradiol (E2) and unlabeled Mini Samples Estradiol (E2) (Standards or samples) with the pre-coated antibody specific to Mini Samples Estradiol (E2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Estradiol (E2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Estradiol (E2) in the sample.

Alternative Names

17B-Estradiol; Oestradiol; Beta-Estradiol

Item Name

Estradiol

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Reference