Mini Samples ELISA Kit for Eosinophil Chemotactic Factor (ECF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than

Sample type

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Eosinophil Chemotactic Factor (ECF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Eosinophil Chemotactic Factor (ECF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Eosinophil Chemotactic Factor (ECF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Eosinophil Chemotactic Factor (ECF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11

Item Name

Eosinophil Chemotactic Factor

Research Area

Cytokine;Infection immunity;Autoimmunity;

Reference