Mini Samples ELISA Kit for Cyclic Guanosine Monophosphate (cGMP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 49.6pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Cyclic Guanosine Monophosphate (cGMP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Cyclic Guanosine Monophosphate (cGMP) and unlabeled Mini Samples Cyclic Guanosine Monophosphate (cGMP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Cyclic Guanosine Monophosphate (cGMP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Cyclic Guanosine Monophosphate (cGMP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Cyclic Guanosine Monophosphate (cGMP) in the sample.

Alternative Names

c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate

Item Name

Cyclic Guanosine Monophosphate

Research Area

Signal transduction;Metabolic pathway;

Reference