Mini Samples ELISA Kit for Cross Linked C-Telopeptide Of Type II Collagen (CTXII)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

98.8-8,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 39.4pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and unlabeled Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) (Standards or samples) with the pre-coated antibody specific to Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample.

Alternative Names

CTX-II; CTX2

Item Name

Cross Linked C-Telopeptide Of Type II Collagen

Research Area

Metabolic pathway;Bone metabolism;Rheumatology;

Reference