Mini Samples ELISA Kit for Creatine Kinase B (CK-BB)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than

Sample type

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Creatine Kinase B (CK-BB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Creatine Kinase B (CK-BB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Creatine Kinase B (CK-BB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Creatine Kinase B (CK-BB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CKB; CKBB; CK-B; CK-1; Creatine Kinase, Brain

Item Name

Creatine Kinase B

Research Area

Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Neuro science;

Reference