Mini Samples ELISA Kit for Corticotropin Releasing Hormone Binding Protein (CRHBP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

62.5-4,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 22.2pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Corticotropin Releasing Hormone Binding Protein (CRHBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Corticotropin Releasing Hormone Binding Protein (CRHBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Corticotropin Releasing Hormone Binding Protein (CRHBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Corticotropin Releasing Hormone Binding Protein (CRHBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CRF-BP; CRFBP; Corticotropin-Releasing Factor-Binding Protein

Item Name

Corticotropin Releasing Hormone Binding Protein

Research Area

Endocrinology;Neuro science;Hormone metabolism;

Reference