Mini Samples ELISA Kit for Corticotropin Releasing Factor (CRF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.37pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Corticotropin Releasing Factor (CRF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Corticotropin Releasing Factor (CRF) and unlabeled Mini Samples Corticotropin Releasing Factor (CRF) (Standards or samples) with the pre-coated antibody specific to Mini Samples Corticotropin Releasing Factor (CRF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Corticotropin Releasing Factor (CRF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Corticotropin Releasing Factor (CRF) in the sample.

Alternative Names

CRH; Corticotropin Releasing Hormone; Corticoliberin

Item Name

Corticotropin Releasing Factor

Research Area

Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;

Reference