Mini Samples ELISA Kit for Brain Natriuretic Peptide (BNP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

6.17-500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.52pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Brain Natriuretic Peptide (BNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Brain Natriuretic Peptide (BNP) and unlabeled Mini Samples Brain Natriuretic Peptide (BNP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Brain Natriuretic Peptide (BNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Brain Natriuretic Peptide (BNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Brain Natriuretic Peptide (BNP) in the sample.

Alternative Names

GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

Item Name

Brain Natriuretic Peptide

Research Area

Cardiovascular biology;Neuro science;Hormone metabolism;

Reference