MBP Monoclonal Antibody

Size

100ug

Clone Number

Aliases

Maltose Binding Protein, MBP, MBP Tag

Immunogen Species

UniProt ID

Immunogen

MBP-Tag fusion protein

Raised in

Mouse

Species Reactivity

All

Tested Applications

ELISA, WB; Recommended dilution:WB:1:500-1:5000

Background

Maltose-Binding Protein (MBP) is a native E. coli protein and is part of a complex responsible for the uptake and efficient catabolism of maltodextrins. MBP is composed of 370 amino acid residues. As a fusion tag, MBP is useful for recombinant protein purification by affinity chromatography. It is also used to increase the expression level and solubility of the tagged protein, and for promoting proper folding of the attached protein. MBP increases solubility and the tag is thus particularly useful for recombinant proteins that may otherwise accumulate in an insoluble forms as inclusion bodies. Affinity purification takes place under physiological conditions and mild elution is performed using maltose. The mild elution conditions preserve the activity of the MBP-tagged protein.

Clonality

Monoclonal

Isotype

IgG2a

Purification Method

>95%,Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

MBP

Research Areas