MAP2K4 Antibody

Size

100ug

Clone Number

Aliases

c Jun N terminal kinase kinase 1 antibody; C-JUN N-terminal kinase kinase 1 antibody; Dual specificity mitogen activated protein kinase kinase 4 antibody; Dual specificity mitogen-activated protein kinase kinase 4 antibody; JNK Activated Kinase 1 antibody; JNK activating kinase 1 antibody; JNK-activating kinase 1 antibody; JNKK antibody; JNKK1 antibody; MAP kinase kinase 4 antibody; Map2k4 antibody; MAPK ERK kinase 4 antibody; MAPK/ERK kinase 4 antibody; MAPKK 4 antibody; MAPKK4 antibody; MEK 4 antibody; MEK4 antibody; Mitogen activated protein kinase kinase 4 antibody; MKK 4 antibody; MKK4 antibody; MP2K4_HUMAN antibody; PRKMK4 antibody; SAPK ERK kinase 1 antibody; SAPK/ERK kinase 1 antibody; SAPKK 1 antibody; SAPKK1 antibody; SEK1 antibody; SERK1 antibody; SKK1 antibody; Stress activated protein kinase kinase 1 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

P45985

Immunogen

Recombinant Human Dual specificity mitogen-activated protein kinase kinase 4 protein (259-398AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500

Background

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

MAP2K4

Research Areas

Signal transduction