LIN28A Antibody

Size

100ug

Clone Number

Aliases

AL024421 antibody; CSDD1 antibody; CSDD2 antibody; FLJ12457 antibody; Lin 28 antibody; Lin 28 homolog (C. elegans) antibody; Lin 28 homolog A (C. elegans) antibody; Lin 28 homolog A antibody; Lin 28 homolog antibody; Lin-28A antibody; Lin28 antibody; Lin28, C. elegans, homolog of, A antibody; LIN28A antibody; LN28A_HUMAN antibody; Protein lin-28 homolog A antibody; Protein lin-28 homolog B antibody; RNA binding protein lin 28 antibody; Tex17 antibody; ZCCHC1 antibody; Zinc finger CCHC domain containing 1 antibody; Zinc finger CCHC domain containing protein 1 antibody; Zinc finger CCHC domain-containing protein 1 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9H9Z2

Immunogen

Recombinant Human Protein lin-28 homolog A protein (1-209AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

Background

RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). \'Translational enhancer\' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5\'-GGAG-3\' motif found in pre-miRNA terminal loop, and recruits ZCCHC11/TUT4 uridylyltransferase. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (By similarity).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

LIN28A

Research Areas

Epigenetics and Nuclear Signaling; Neuroscience; Developmental biology; Stem cells