lexA Antibody

Size

100ug

Clone Number

Aliases

lexA antibody; exrA antibody; spr antibody; tsl antibody; umuA antibody; b4043 antibody; JW4003LexA repressor antibody; EC 3.4.21.88 antibody

Immunogen Species

Escherichia coli

UniProt ID

P0A7C2

Immunogen

Recombinant Escherichia coli LexA repressor protein (1-202AA)

Raised in

Rabbit

Species Reactivity

Escherichia coli

Tested Applications

ELISA, WB; Recommended dilution: WB:1:500-1:5000

Background

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5\\\'-CTGTATATATATACAG-3\\\'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

lexA

Research Areas

Others