KLHL20 Antibody

Size

100ug

Clone Number

Aliases

Kelch like ECT2 interacting protein antibody; Kelch like protein 20 antibody; Kelch like protein X antibody; Kelch motif containing protein antibody; Kelch-like ECT2-interacting protein antibody; Kelch-like protein 20 antibody; Kelch-like protein X antibody; KHLHX antibody; KLEIP antibody; KLH20_HUMAN antibody; KLHL20 antibody; KLHLX antibody; RP3-383J4.3 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9Y2M5

Immunogen

Recombinant Human Kelch-like protein 20 protein (1-200AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200

Background

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis (PubMed:20389280). The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates \\\'Lys-33\\\'-linked ubiquitination (PubMed:24768539). Involved in anterograde Golgi to endosome transport by mediating \\\'Lys-33\\\'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking (PubMed:24768539). Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (PubMed:21670212). In case of tumor, the BCR(KLHL20) E3 ubiquitin ligase complex is involved in tumor hypoxia: following hypoxia, the BCR(KLHL20)complex mediates ubiquitination and degradation of PML, potentiating HIF-1 signaling and cancer progression (PubMed:21840486).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

KLHL20

Research Areas

Cardiovascular; Metabolism; Signal transduction