KDM1A Antibody

Size

100ug

Clone Number

Aliases

Amine oxidase (flavin containing) domain 2 antibody; Amine oxidase, flavin containing, 2 antibody; AOF2 antibody; BHC110 antibody; BRAF35 HDAC complex protein BHC110 antibody; BRAF35-HDAC complex protein BHC110 antibody; BRAF35/HDAC complex, 110-kD subunit antibody; CPRF antibody; EC1 antibody; FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody; Flavin-containing amine oxidase domain-containing protein 2 antibody; KDM 1 antibody; KDM1 antibody; Kdm1a antibody; KDM1A_HUMAN antibody; KIAA0601 antibody; LSD 1 antibody; LSD1 antibody; Lysine (K) specific demethylase 1 antibody; Lysine (K) specific demethylase 1A antibody; Lysine demethylase 1A antibody; Lysine specific histone demethylase 1 antibody; Lysine specific histone demethylase 1A antibody; Lysine-specific demethylase 1 antibody; Lysine-specific demethylase 1A antibody; Lysine-specific histone demethylase 1A antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

O60341

Immunogen

Recombinant Human Lysine-specific histone demethylase 1A protein (100-150AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Background

Histone demethylase that demethylates both \\\'Lys-4\\\' (H3K4me) and \\\'Lys-9\\\' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of \\\'Thr-6\\\' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated \\\'Lys-370\\\' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

KDM1A

Research Areas

Epigenetics and Nuclear Signaling