ISCU Antibody

Size

100ul

Clone Number

Aliases

2310020H20Rik antibody; HML antibody; hnifU antibody; Iron sulfur cluster assembly enzyme ISCU mitochondrial antibody; Iron sulfur cluster scaffold homolog (E. coli) antibody; Iron sulfur cluster scaffold homolog antibody; Iron-sulfur cluster assembly enzyme ISCU antibody; Iscu antibody; IscU iron sulfur cluster scaffold homolog antibody; ISCU_HUMAN antibody; ISU2 antibody; MGC74517 antibody; mitochondrial antibody; NIFU antibody; NifU like N terminal domain containing antibody; NifU like N terminal domain containing protein antibody; NifU like protein antibody; NifU-like N-terminal domain-containing protein antibody; NifU-like protein antibody; NIFUN antibody; Nitrogen fixation cluster like antibody; OTTHUMP00000238760 antibody; OTTHUMP00000238761 antibody; OTTHUMP00000238762 antibody; OTTHUMP00000238764 antibody; OTTHUMP00000238765 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9H1K1

Immunogen

Recombinant Human Iron-sulfur cluster assembly enzyme ISCU, mitochondrial protein (1-167AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Background

Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity).

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

ISCU

Research Areas

Metabolism; Signal transduction