IRAK4 Antibody

Size

100ul

Clone Number

Aliases

IL-1 receptor-associated kinase 4 antibody; Interleukin 1 receptor associated kinase 4 mutant form 1 antibody; Interleukin-1 receptor-associated kinase 4 antibody; Interleukin1 receptor associated kinase 4 antibody; IPD1 antibody; IRAK 4 antibody; IRAK-4 antibody; IRAK4 antibody; IRAK4 mutated form 1 antibody; IRAK4_HUMAN antibody; LOC 51135 antibody; NY REN 64 antibody; NY REN 64 antigen antibody; NY-REN-64 antibody; REN64 antibody; Renal carcinoma antigen NY-REN-64 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9NWZ3

Immunogen

Recombinant Human Interleukin-1 receptor-associated kinase 4 protein (1-220AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA

Background

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

IRAK4

Research Areas

Cardiovascular; Immunology; Signal transduction