Instant ELISA Kit for Thromboxane B2 (TXB2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

18.52-1,500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 7.14pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

1h, 10min

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Instant Thromboxane B2 (TXB2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Instant Thromboxane B2 (TXB2) and unlabeled Instant Thromboxane B2 (TXB2) (Standards or samples) with the pre-coated antibody specific to Instant Thromboxane B2 (TXB2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Instant Thromboxane B2 (TXB2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Instant Thromboxane B2 (TXB2) in the sample.

Alternative Names

TX-B2

Item Name

Thromboxane B2

Research Area

Metabolic pathway;Infection immunity;

Reference