Instant ELISA Kit for S100 Calcium Binding Protein B (S100B)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.625-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.21ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

1h, 10min

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant S100 Calcium Binding Protein B (S100B). Standards or samples and HRP-labeled detection antibody specific to Instant S100 Calcium Binding Protein B (S100B) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant S100 Calcium Binding Protein B (S100B), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant S100 Calcium Binding Protein B (S100B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

S100-B; NEF; S100Beta; S-100 protein subunit beta

Item Name

S100 Calcium Binding Protein B

Research Area

Tumor immunity;Infection immunity;Neuro science;

Reference