Instant ELISA Kit for Isocitrate Dehydrogenase 1, Soluble (IDH1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.5-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.7ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

1h, 10min

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Isocitrate Dehydrogenase 1, Soluble (IDH1). Standards or samples and HRP-labeled detection antibody specific to Instant Isocitrate Dehydrogenase 1, Soluble (IDH1) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Isocitrate Dehydrogenase 1, Soluble (IDH1), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Isocitrate Dehydrogenase 1, Soluble (IDH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase

Item Name

Isocitrate Dehydrogenase 1, Soluble

Research Area

Enzyme & Kinase;

Reference