Instant ELISA Kit for Interleukin 8 (IL8)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Chicken (Gallus)

Sensitivity

The minimum detectable dose of this kit is typically less than

Sample type

Assay length

1h, 10min

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Interleukin 8 (IL8). Standards or samples and HRP-labeled detection antibody specific to Instant Interleukin 8 (IL8) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Interleukin 8 (IL8), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

Item Name

Interleukin 8

Research Area

Cytokine;Infection immunity;

Reference