Instant ELISA Kit for Integrin Beta 1 (ITGb1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.78-50ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.31ng/mL

Sample type

Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

1h, 10min

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Integrin Beta 1 (ITGb1). Standards or samples and HRP-labeled detection antibody specific to Instant Integrin Beta 1 (ITGb1) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Integrin Beta 1 (ITGb1), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Integrin Beta 1 (ITGb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CD29; MSK12; ITG-B1; FNRB; GPIIA; MDF2; MSK12; VLAB; Fibronectin Receptor Beta Polypeptide; Antigen CD29 Includes MDF2; Glycoprotein IIa; VLA-4 subunit beta

Item Name

Integrin Beta 1

Research Area

CD & Adhesion molecule;Tumor immunity;

Reference