Instant ELISA Kit for Indole 3 Acetic Acid (IAA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

320-200,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 110pg/mL

Sample type

Tissue or cell culture supernates

Assay length

1h, 10min

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Instant Indole 3 Acetic Acid (IAA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Instant Indole 3 Acetic Acid (IAA) and unlabeled Instant Indole 3 Acetic Acid (IAA) (Standards or samples) with the pre-coated antibody specific to Instant Indole 3 Acetic Acid (IAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Instant Indole 3 Acetic Acid (IAA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Instant Indole 3 Acetic Acid (IAA) in the sample.

Alternative Names

IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin

Item Name

Indole 3 Acetic Acid

Research Area

Signal transduction;Hormone metabolism;

Reference