Instant ELISA Kit for High Mobility Group Protein 1 (HMGB1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

62.5-4,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 22.5pg/mL

Sample type

serum, plasma and other biological fluids

Assay length

1h, 10min

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant High Mobility Group Protein 1 (HMGB1). Standards or samples and HRP-labeled detection antibody specific to Instant High Mobility Group Protein 1 (HMGB1) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant High Mobility Group Protein 1 (HMGB1), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant High Mobility Group Protein 1 (HMGB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein

Item Name

High Mobility Group Protein 1

Research Area

Infection immunity;

Reference