Instant ELISA Kit for Growth Differentiation Factor 5 (GDF5)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

15.6-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 6.2pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

1h, 10min

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Growth Differentiation Factor 5 (GDF5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Instant Growth Differentiation Factor 5 (GDF5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Instant Growth Differentiation Factor 5 (GDF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Growth Differentiation Factor 5 (GDF5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CDMP1; LAP4; SYNS2; BMP14; Radotermin; Cartilage-Derived Morphogenetic Protein-1; Bone morphogenetic protein 14; Lipopolysaccharide-associated protein 4

Item Name

Growth Differentiation Factor 5

Research Area

Cytokine;Signal transduction;Neuro science;Developmental science;Bone metabolism;

Reference