Instant ELISA Kit for Free Thyroxine (fT4)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than

Sample type

Assay length

1h, 10min

Method

Competitive Inhibition

Test principle

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Free Thyroxine (fT4). Standards or samples and HRP-labeled detection antibody specific to Instant Free Thyroxine (fT4) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Free Thyroxine (fT4), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Free Thyroxine (fT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

FTI

Item Name

Free Thyroxine

Research Area

Reference