Instant ELISA Kit for Estradiol (E2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.65pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

1h, 10min

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Instant Estradiol (E2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Instant Estradiol (E2) and unlabeled Instant Estradiol (E2) (Standards or samples) with the pre-coated antibody specific to Instant Estradiol (E2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Instant Estradiol (E2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Instant Estradiol (E2) in the sample.

Alternative Names

17B-Estradiol; Oestradiol; Beta-Estradiol

Item Name

Estradiol

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Reference