HPSE Antibody

Size

100ug

Clone Number

Aliases

Endo glucoronidase antibody; Endo-glucoronidase antibody; HEP antibody; Heparanase 50 kDa subunit antibody; Heparanase antibody; Heparanase-1 antibody; Heparanase1 antibody; Hpa 1 antibody; HPA antibody; Hpa1 antibody; HPR 1 antibody; HPR1 antibody; HPSE 1 antibody; HPSE antibody; HPSE_HUMAN antibody; HPSE1 antibody; HSE 1 antibody; HSE1 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9Y251

Immunogen

Recombinant Human Heparanase protein (263-523AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200

Background

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

HPSE

Research Areas

Cancer; Tags & Cell Markers; Signal transduction