Histone H3.3 Antibody

Size

100ul

Clone Number

4H2

Aliases

Histone H3.3, H3F3A, H3.3A, H3F3, PP781, AND, H3F3B, H3.3B

Immunogen Species

Human

UniProt ID

P84243

Immunogen

A synthesized peptide

Raised in

Species Reactivity

Human

Tested Applications

ELISA, IHC, IF; Recommended dilution: IHC:1:50-1:500, IF:1:30-1:200

Background

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Clonality

Monoclonal

Isotype

Rabbit IgG

Purification Method

Affinity-chromatography

Conjugate

Non-conjugated

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

H3F3A

Research Areas

Epigenetics and Nuclear Signaling