High Sensitive ELISA Kit for Trefoil Factor 3 (TFF3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

46.88-3000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 19.11pg/mL

Sample type

serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Trefoil Factor 3 (TFF3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Trefoil Factor 3 (TFF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Trefoil Factor 3 (TFF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Trefoil Factor 3 (TFF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

HITF; ITF3; TFI; HP1.B; Trefoil Factor 3, Intestinal

Item Name

Trefoil Factor 3

Research Area

Tumor immunity;Gastroenterology;

Reference