High Sensitive ELISA Kit for Testosterone (Testo)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.65pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Testosterone (Testo) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Testosterone (Testo) and unlabeled High Sensitive Testosterone (Testo) (Standards or samples) with the pre-coated antibody specific to High Sensitive Testosterone (Testo). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Testosterone (Testo) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Testosterone (Testo) in the sample.

Alternative Names

T; Testesteron; Testeronun

Item Name

Testosterone

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Reference

Revisiting the relationships of 2D:4D with androgen receptor (AR) gene and current testosterone levels: Replication study and meta‐analyses;