High Sensitive ELISA Kit for Reverse Triiodothyronine (rT3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

24.69-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 9.04pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Reverse Triiodothyronine (rT3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Reverse Triiodothyronine (rT3) and unlabeled High Sensitive Reverse Triiodothyronine (rT3) (Standards or samples) with the pre-coated antibody specific to High Sensitive Reverse Triiodothyronine (rT3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Reverse Triiodothyronine (rT3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Reverse Triiodothyronine (rT3) in the sample.

Alternative Names

RT3; r-T3; Reverse T3;(2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine

Item Name

Reverse Triiodothyronine

Research Area

Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity;

Reference