High Sensitive ELISA Kit for Neuropeptide Y (NPY)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.47-200pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.94pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Neuropeptide Y (NPY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Neuropeptide Y (NPY) and unlabeled High Sensitive Neuropeptide Y (NPY) (Standards or samples) with the pre-coated antibody specific to High Sensitive Neuropeptide Y (NPY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Neuropeptide Y (NPY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Neuropeptide Y (NPY) in the sample.

Alternative Names

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

Item Name

Neuropeptide Y

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hepatology;

Reference