High Sensitive ELISA Kit for Neurofilament, Light Polypeptide (NEFL)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

4.94-400pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.95pg/mL

Sample type

Serum, plasma, cerebrospinal fluid and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Neurofilament, Light Polypeptide (NEFL) and unlabeled High Sensitive Neurofilament, Light Polypeptide (NEFL) (Standards or samples) with the pre-coated antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the sample.

Alternative Names

CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein

Item Name

Neurofilament, Light Polypeptide

Research Area

Neuro science;

Reference