High Sensitive ELISA Kit for Interleukin 6 (IL6)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.56-100pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Bos taurus; Bovine (Cattle)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.49pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor

Item Name

Interleukin 6

Research Area

Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology;

Reference

Dynamics of salivary immunoglobulin A and serum interleukin 6 levels in newborn calves;Serum interleukin-6 as a prognostic marker in neonatal calf diarrhea;Cytokines and immunoglobulin genetics in neonatal calf diarrhea;Candidates present views;