High Sensitive ELISA Kit for Hepcidin (Hepc)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 87.3pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Hepcidin (Hepc) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Hepcidin (Hepc) and unlabeled High Sensitive Hepcidin (Hepc) (Standards or samples) with the pre-coated antibody specific to High Sensitive Hepcidin (Hepc). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Hepcidin (Hepc) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Hepcidin (Hepc) in the sample.

Alternative Names

HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor

Item Name

Hepcidin

Research Area

Metabolic pathway;Endocrinology;Hematology;Genetic science;

Reference