High Sensitive ELISA Kit for Epinephrine (EPI)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

6.17-500pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.54pg/mL

Sample type

Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Epinephrine (EPI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Epinephrine (EPI) and unlabeled High Sensitive Epinephrine (EPI) (Standards or samples) with the pre-coated antibody specific to High Sensitive Epinephrine (EPI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Epinephrine (EPI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Epinephrine (EPI) in the sample.

Alternative Names

Adrenaline

Item Name

Epinephrine

Research Area

Metabolic pathway;Infection immunity;Neuro science;Hormone metabolism;

Reference