High Sensitive ELISA Kit for Cardiac Troponin I (cTnI)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.12-200pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.29pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Cardiac Troponin I (cTnI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Cardiac Troponin I (cTnI) and unlabeled High Sensitive Cardiac Troponin I (cTnI) (Standards or samples) with the pre-coated antibody specific to High Sensitive Cardiac Troponin I (cTnI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Cardiac Troponin I (cTnI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Cardiac Troponin I (cTnI) in the sample.

Alternative Names

TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac

Item Name

Cardiac Troponin I

Research Area

Cardiovascular biology;

Reference