High Sensitive ELISA Kit for Advanced Glycation End Product (AGE)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

19.75-1,600ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 7.13ng/mL

Sample type

serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Advanced Glycation End Product (AGE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Advanced Glycation End Product (AGE) and unlabeled High Sensitive Advanced Glycation End Product (AGE) (Standards or samples) with the pre-coated antibody specific to High Sensitive Advanced Glycation End Product (AGE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Advanced Glycation End Product (AGE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Advanced Glycation End Product (AGE) in the sample.

Alternative Names

AGEs; Advanced Glycation End Products

Item Name

Advanced Glycation End Product

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Hormone metabolism;

Reference