GFP Monoclonal Antibody

Size

100ug

Clone Number

Aliases

CFP, eGFP, eYFP, GFP, GFP tag, YFP

Immunogen Species

UniProt ID

Immunogen

Recombinant GFP Protein

Raised in

Mouse

Species Reactivity

All

Tested Applications

ELISA, WB, IP; Recommended dilution:WB:1:500-1:5000, IP:1:200-1:2000

Background

The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.

Clonality

Monoclonal

Isotype

IgG2b

Purification Method

>95%,Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

GFP

Research Areas