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FPR003-0013

Flip Mycoplasma

Flip Mycoplasma

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⚡ 12-minute result · 🧪 PCR-grade performance (target) · ✅ Visual readout

Flip Mycoplasma

A ready-to-use, single-use nucleic acid amplification test designed to detect mycoplasma contamination in cell culture with a 12-minute sample-to-result workflow. (Target: 16S rRNA)


Ready-to-use Ambient storage Hands-on < 1 min 50°C incubation
For Research Use Only (RUO). Not for diagnostic procedures.
Flip Mycoplasma kit image

Why mycoplasma testing matters

Contamination can be “silent”
Mycoplasma are small, lack a cell wall, and can persist in cultures while impacting cell physiology, assay reproducibility, and downstream product quality.
What Flip Mycoplasma helps with
  • Ultra-fast: 12-minute sample-to-result; hands-on time < 1 minute
  • Sensitive: stated LoD of 25 CFU/mL (manufacturer claim)
  • Simple: no freezer required; direct sample testing; visual readout

Key features

Accurate
Targets 16S rRNA to support robust specificity; LoD stated as 25 CFU/mL.
Ultra-rapid
Designed for PCR-grade performance with a 12-minute workflow—easy to routine.
Simple & flexible
Runs on a 0.2 mL heat block or thermal cycler at 50°C. Optional USB-C powered Flip Nest.
Item Details
Target Qualitative (Yes/No) detection of mycoplasma via 16S rRNA
Time 12 minutes sample-to-result; hands-on < 1 minute
Incubation 50°C for 10 minutes, then flip and read (per manufacturer guidance)
Storage Lyophilised format; ambient storage. If chilled, allow to return to room temperature before use.
Readout Visual line readout with an internal process control line (validity / inhibition check)
Notes: Performance depends on sample matrix and workflow. Specifications above reflect manufacturer-provided information.

Workflow (overview)

For full instructions, refer to the manufacturer’s Quick Use Guide / IFU.
Prepare sample
Collect cell-culture supernatant and dilute with the supplied Sample Buffer to 10% (v/v).
Incubate (50°C)
Pre-warm a 0.2 mL heat block or thermal cycler to 50°C. Incubate the assembled test for exactly 10 minutes.
Flip & read
Flip per instructions, confirm fluid flow on the strip, then read results after ~2 minutes.

Sample types & prep tips

  • Cell culture supernatant: suitable for suspension and adherent cultures (test before passaging/media change).
  • FBS screening: check serum lots before use in critical workflows.
  • Suspension / semi-adherent: remove cells by centrifugation (500 g, 5 min) and test supernatant (per manufacturer guidance).
Highly confluent, long-maintained cultures may accumulate inhibitors that can affect assay performance (manufacturer note).

What you need

Basics
Pipette & tips, 1.5 mL tubes, timer
Heating
0.2 mL heat block or thermal cycler (50°C)
If needed
Microcentrifuge (to clear cells from some samples)
Water baths are not recommended due to contamination risk (manufacturer guidance).

Pack sizes & ordering

Manufacturer-listed pack size options: 10 tests / 50 tests / 100 tests
External Positive Control (optional)
Useful for onboarding/training and troubleshooting. Available as a separate product.
Request a quote
To speed things up, share:
  • Expected tests per month/quarter
  • Main sample types (supernatant / FBS) and media composition
  • Available equipment (50°C heat block / thermal cycler)
  • Ship-to address and PO/invoicing needs
Pricing and lead time may vary by region and distributor policy.

Documents (manufacturer)

Document links may change based on the manufacturer’s site policy.
SEO keywords
Mycoplasma test, mycoplasma contamination, cell culture QC, rapid molecular test, 16S rRNA, nucleic acid amplification, ambient storage kit, 50°C heat block, PCR alternative, FBS screening, RUO

Product: Flip Mycoplasma · Manufacturer: Fuse Diagnostics (Flip DX). Trademarks belong to their respective owners.
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