Flag Tag Monoclonal Antibody

Size

100ul

Clone Number

4G5C8

Aliases

DDK tag, DYKDDDDK, Flag, Flag Tag

Immunogen Species

UniProt ID

Immunogen

DYKDDDDK(Flag) synthetic peptide conjugate to KLH

Raised in

Mouse

Species Reactivity

Tested Applications

ELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl

Background

Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

Clonality

Monoclonal

Isotype

IgG2a

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

Flag Tag

Research Areas