FAN1 Antibody

Size

100ug

Clone Number

Aliases

6030441H18Rik antibody; Coiled coil domain containing protein MTMR15 antibody; DKFZp451H236 antibody; DKFZp686K16147 antibody; FAN1 antibody; FAN1_HUMAN antibody; FANCD2 and FANCI associated nuclease 1 antibody; FANCD2/FANCI associated nuclease 1 antibody; FANCD2/FANCI-associated nuclease 1 antibody; Fanconi associated nuclease 1 antibody; Fanconi-associated nuclease 1 antibody; hFAN1 antibody; KIAA1018 antibody; KMIN antibody; MTMR 15 antibody; MTMR15 antibody; Myotubularin related protein 15 antibody; Myotubularin-related protein 15 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9Y2M0

Immunogen

Recombinant Human Fanconi-associated nuclease 1 protein (167-373AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500

Background

Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771). Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016). Acts as a 5\\\'-3\\\' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3\\\'-flap annealing until it reaches and unhooks the ICL (PubMed:25430771). Acts at sites that have a 5\\\'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3\\\' flap (PubMed:25430771). Also has endonuclease activity toward 5\\\'-flaps (PubMed:20603015, PubMed:20603016, PubMed:24981866).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

FAN1

Research Areas

Epigenetics and Nuclear Signaling