Perforin 1 rabbit pAb

Applications: WB;IHC;ELISA
Reactivity: Human;Rat;Mouse;
Source: Rabbit
Dilution: WB 1:500-2000, IHC 1:50-200, ELISA 1:10000-20000
Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human PRF1. AA range:451-500
Storage_stability: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed_band(KD): 61kD
Human_gene_id: 5551
Human_swiss_prot_no: P14222
Subcellular_location: Cytolytic granule . Secreted. Cell membrane ; Multi-pass membrane protein . Endosome lumen . Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell (PubMed:20038786). Inserts into the cell membrane of target cells and forms pores (PubMed:20889983). Membrane insertion and pore formation requires a major conformation change (PubMed:20889983). May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes (PubMed:20038786). .
Other_name: Perforin-1 (P1) (Cytolysin) (Lymphocyte pore-forming protein) (PFP)
Background: The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],