Crk II (phospho Tyr221) rabbit pAb

Applications: WB;IF;ELISA
Reactivity: Human;Mouse;Rat;Monkey
Source: Rabbit
Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen: The antiserum was produced against synthesized peptide derived from human CrkII around the phosphorylation site of Tyr221. AA range:187-236
Storage_stability: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed_band(KD): 40kD
Human_gene_id: 1398
Human_swiss_prot_no: P46108
Subcellular_location: Cytoplasm . Cell membrane . Translocated to the plasma membrane upon cell adhesion. .
Other_name: CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38
Background: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008],