SLP-76 (phospho-Ser376) rabbit pAb

Applications: WB;ELISA;IHC
Reactivity: Human;Mouse
Source: Rabbit
Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen: Synthesized phosho peptide around human SLP-76 (Ser376)
Storage_stability: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed_band(KD): 75kD
Human_gene_id: 3937
Human_swiss_prot_no: Q13094
Subcellular_location: Cytoplasm .
Other_name: Lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of 76 kDa) (SLP-76 tyrosine phosphoprotein) (SLP76)
Background: SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T c