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ELK Biotechnology
SKU:ES1076
His-tag rabbit pAb
His-tag rabbit pAb
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$250.00 USD
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$250.00 USD
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Applications: WB;ELISA
Reactivity: Species independent
Source: Rabbit
Dilution: Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen: 6X His synthetic peptide conjugated to KLH.
Storage_stability: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed_band(KD): 15kD
Other_name: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Background: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
Reactivity: Species independent
Source: Rabbit
Dilution: Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen: 6X His synthetic peptide conjugated to KLH.
Storage_stability: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed_band(KD): 15kD
Other_name: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Background: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
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