MRCKG rabbit pAb

Applications: WB;ELISA
Reactivity: Human;Rat;Mouse;
Source: Rabbit
Dilution: WB 1:500-2000 ELISA 1:5000-20000
Immunogen: Synthesized peptide derived from human protein . at AA range: 1370-1450
Storage_stability: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed_band(KD): 170kD
Human_gene_id: 55561
Human_swiss_prot_no: Q6DT37
Subcellular_location: Cytoplasm . Concentrates at the leading edge of cells.
Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation.,function:May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 CNH domain.,similarity:Contains 1 CRIB domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,subcellular location:Concentrates at the leading edge of cells.,subunit:Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42.,tissue specificity:Expressed in heart and skeletal muscle.,