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ELK Biotechnology
SKU:EM1031
β-actin Mouse mAb
β-actin Mouse mAb
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$250.00 USD
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Applications: WB, IHC, IF
Reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep
Source: Mouse
Dilution: WB 1:5,000-10,000 IHC 1:200 IF 1:200
Immunogen: Synthetic Peptide
Storage_stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/mL
Observed_band(KD): 43kDa
Human_gene_id: 60
Human_swiss_prot_no: P60709
Subcellular_location: Cytoplasm,Cytoskeleton
Other_name: actin antibody, b-actin antibody, beta-actin antibody
Background: β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
Reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep
Source: Mouse
Dilution: WB 1:5,000-10,000 IHC 1:200 IF 1:200
Immunogen: Synthetic Peptide
Storage_stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/mL
Observed_band(KD): 43kDa
Human_gene_id: 60
Human_swiss_prot_no: P60709
Subcellular_location: Cytoplasm,Cytoskeleton
Other_name: actin antibody, b-actin antibody, beta-actin antibody
Background: β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
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