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ELK Biotechnology
SKU:ELK9947
Human SV2A(Synaptic Vesicle Glycoprotein 2A) ELISA Kit
Human SV2A(Synaptic Vesicle Glycoprotein 2A) ELISA Kit
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Alternative Names: SV2A
Assay Type: Sandwich
Sensitivity: 0.069 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SV2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SV2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SV2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SV2A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q7L0J3
Assay Type: Sandwich
Sensitivity: 0.069 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SV2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SV2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SV2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SV2A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q7L0J3
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